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aalagarosebeads  (Vector Laboratories)


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    Structured Review

    Vector Laboratories aalagarosebeads
    Aalagarosebeads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AL-1393-2/Agarose+bound+Aleuria+Aurantia+Lectin+(AAL)/pm40295503-404-5-6
    Average 93 stars, based on 30 article reviews
    aalagarosebeads - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Fucosyltransferase 5 Promotes the Proliferative and Migratory Properties of Intrahepatic Cholangiocarcinoma Cells via Regulating Protein Glycosylation Profiles
    Article Snippet: Goat anti-rabbit IgG and goat anti-mouse IgG secondary antibodies were purchased from Beijing Solarbio Science and Technology Co., Ltd. .. Next, 100 μL of agarose-bound Aleuria Aurantia Lectin (AL-1393-2, Vector Laboratories) was added to 400 μg of total cell lysate and incubated at 4°C overnight on a rotator. α1,3-fucosylated glycoproteins were enriched by centrifugation and washed thrice with HBS buffer. ..

    Article Title: Structure and dynamics of the Arabidopsis O-fucosyltransferase SPINDLY.
    Article Snippet: After this, 20 μL of each protein extract was mixed with 30 μL of 2× Laemmli Sample Buffer (LSB) (Bio-Rad) for input analysis. .. To 1.5mL of extract, 20 μL of AALagarose beads (Vector Labs, AL-1393-2, 2mg lectin/mL) were added and incubated with rotation for 1.5 h at 4 °C. ..

    Article Title: O-Fucosyltransferase SPINDLY attenuates auxin-induced fruit growth by inhibiting ARF6/8-coactivator mediator complex interaction in Arabidopsis.
    Article Snippet: .. Protein extracts were incubated with AALagarosebeads (Vector Labs, AL-1393-2, 2mg lectin/mL) to enrich forOfucosylated proteins and analyzed by immunoblot analysis as described34. ..

    Article Title: The O -Fucosyltransferase SPINDLY Attenuates Auxin-Induced Fruit Growth by Inhibiting ARF6 and ARF8 binding to Coactivator Mediator Complex in Arabidopsis
    Article Snippet: .. Protein extracts were incubated with AAL-agarose beads (Vector Labs, AL-1393-2, 2 mg lectin/mL) to enrich for O -fucosylated proteins, and analyzed by immunoblot analysis as described . ..

    Article Title: O -Fucosyltransferase SPINDLY attenuates auxin-induced fruit growth by inhibiting ARF6/8-coactivator mediator complex interaction in Arabidopsis
    Article Snippet: .. Protein extracts were incubated with AAL-agarose beads (Vector Labs, AL-1393-2, 2 mg lectin/mL) to enrich for O -fucosylated proteins and analyzed by immunoblot analysis as described . ..

    Centrifugation:

    Article Title: Fucosyltransferase 5 Promotes the Proliferative and Migratory Properties of Intrahepatic Cholangiocarcinoma Cells via Regulating Protein Glycosylation Profiles
    Article Snippet: Goat anti-rabbit IgG and goat anti-mouse IgG secondary antibodies were purchased from Beijing Solarbio Science and Technology Co., Ltd. .. Next, 100 μL of agarose-bound Aleuria Aurantia Lectin (AL-1393-2, Vector Laboratories) was added to 400 μg of total cell lysate and incubated at 4°C overnight on a rotator. α1,3-fucosylated glycoproteins were enriched by centrifugation and washed thrice with HBS buffer. ..

    Western Blot:

    Article Title: O-Fucosyltransferase SPINDLY attenuates auxin-induced fruit growth by inhibiting ARF6/8-coactivator mediator complex interaction in Arabidopsis.
    Article Snippet: .. Protein extracts were incubated with AALagarosebeads (Vector Labs, AL-1393-2, 2mg lectin/mL) to enrich forOfucosylated proteins and analyzed by immunoblot analysis as described34. ..

    Article Title: The O -Fucosyltransferase SPINDLY Attenuates Auxin-Induced Fruit Growth by Inhibiting ARF6 and ARF8 binding to Coactivator Mediator Complex in Arabidopsis
    Article Snippet: .. Protein extracts were incubated with AAL-agarose beads (Vector Labs, AL-1393-2, 2 mg lectin/mL) to enrich for O -fucosylated proteins, and analyzed by immunoblot analysis as described . ..

    Article Title: O -Fucosyltransferase SPINDLY attenuates auxin-induced fruit growth by inhibiting ARF6/8-coactivator mediator complex interaction in Arabidopsis
    Article Snippet: .. Protein extracts were incubated with AAL-agarose beads (Vector Labs, AL-1393-2, 2 mg lectin/mL) to enrich for O -fucosylated proteins and analyzed by immunoblot analysis as described . ..



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    FUT5 can regulate protein glycosylation to promote ICC progression. (A) and (B) Glycosylation sites found by the MS. (C) and (D) Grouped Gene Ontology of the differential glycosylation sites. (E) Several glycosylation sites which downregulate most when FUT5 was knockdown. (F) Volcano plot of protein expression measured by the MS. (G) Protein expression of those in (E) (Several proteins are not identified by MS may because of its low abundance). (H) <t>Lectin</t> enrichment assay shows change of the fucosylation level of ITGB3.
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    Image Search Results


    FUT5 can regulate protein glycosylation to promote ICC progression. (A) and (B) Glycosylation sites found by the MS. (C) and (D) Grouped Gene Ontology of the differential glycosylation sites. (E) Several glycosylation sites which downregulate most when FUT5 was knockdown. (F) Volcano plot of protein expression measured by the MS. (G) Protein expression of those in (E) (Several proteins are not identified by MS may because of its low abundance). (H) Lectin enrichment assay shows change of the fucosylation level of ITGB3.

    Journal: Clinical Medicine Insights. Oncology

    Article Title: Fucosyltransferase 5 Promotes the Proliferative and Migratory Properties of Intrahepatic Cholangiocarcinoma Cells via Regulating Protein Glycosylation Profiles

    doi: 10.1177/11795549231181189

    Figure Lengend Snippet: FUT5 can regulate protein glycosylation to promote ICC progression. (A) and (B) Glycosylation sites found by the MS. (C) and (D) Grouped Gene Ontology of the differential glycosylation sites. (E) Several glycosylation sites which downregulate most when FUT5 was knockdown. (F) Volcano plot of protein expression measured by the MS. (G) Protein expression of those in (E) (Several proteins are not identified by MS may because of its low abundance). (H) Lectin enrichment assay shows change of the fucosylation level of ITGB3.

    Article Snippet: Next, 100 μL of agarose-bound Aleuria Aurantia Lectin (AL-1393-2, Vector Laboratories) was added to 400 μg of total cell lysate and incubated at 4°C overnight on a rotator. α1,3-fucosylated glycoproteins were enriched by centrifugation and washed thrice with HBS buffer.

    Techniques: Knockdown, Expressing