Journal: Clinical Medicine Insights. Oncology
Article Title: Fucosyltransferase 5 Promotes the Proliferative and Migratory Properties of Intrahepatic Cholangiocarcinoma Cells via Regulating Protein Glycosylation Profiles
doi: 10.1177/11795549231181189
Figure Lengend Snippet: FUT5 can regulate protein glycosylation to promote ICC progression. (A) and (B) Glycosylation sites found by the MS. (C) and (D) Grouped Gene Ontology of the differential glycosylation sites. (E) Several glycosylation sites which downregulate most when FUT5 was knockdown. (F) Volcano plot of protein expression measured by the MS. (G) Protein expression of those in (E) (Several proteins are not identified by MS may because of its low abundance). (H) Lectin enrichment assay shows change of the fucosylation level of ITGB3.
Article Snippet: Next, 100 μL of agarose-bound Aleuria Aurantia Lectin (AL-1393-2, Vector Laboratories) was added to 400 μg of total cell lysate and incubated at 4°C overnight on a rotator. α1,3-fucosylated glycoproteins were enriched by centrifugation and washed thrice with HBS buffer.
Techniques: Knockdown, Expressing